Purified Human Liver Acid A - D - Galactosidases Possessing Activity towards GMl - Ganglioside and Lactosylceramide By ARNOLD
نویسنده
چکیده
Our studies with purified human liver acid 6-D-galactosidases (EC 3.2.1.23) indicate that 4-methylumbelliferyl fi-D-galactosidase and GM1-ganglioside /-D-galactosidase activities are identical with lactosylceramidase II activity. Evidence for this includes co-purification of all enzyme activities by affinity chromatography to yield a single band on polyacrylamide-gel electrophoresis and coincident elution from Sepharose 6B of all three enzyme activities.
منابع مشابه
GM1 ganglioside beta-galactosidase. A. Purification and studies of the enzyme from human liver.
GMM1-ganglioside fi-galactosidase A (EC 3.2.1.23) has been purified 17,000-fold from human liver. The enzyme appeared as a single band of protein on polyacrylamide gel electrophoresis. Double immunodiffusion of rabbit anti-pgalactosidase A antiserum against either GM1 /3-galactosidase A or liver supematant gave a single precipitin band. The apparent molecular weight was 65,000 to 75,000 by gel ...
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